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KMID : 0613820070170070948
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Journal of Life Science
2007 Volume.17 No. 7 p.948 ~ p.955
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Effects of AEBSF on the Delay of Spontaneous Apoptosis and the Trans-Differentiation of Human Neutrophils into Dendritic Cells
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Park Hye-Young
Kwak Jong-Young
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Abstract
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Neutrophils play a key role as a first line of defense and are known to acquire the characteristics of dendritic cells (DCs) under the appropriate conditions. The spontaneous apoptosis of neutrophils was delayed by treatment with 4-(2-aminoethyl) benzensulfonylfluoride (AEBSF), a serine protease inhibitor. AEBSF inhibited both caspase-3 and serine protease activities, whereas zVAD-fmk, a pancaspase inhibitor, inhibited only caspase-3 activity. The life span of neutrophils was prolonged up to 5 days by AEBSF in the presence or absence of granulocyte macrophage colony stimulating factor (GM-CSF). DC surface markers, such as CD80, CD83, and MHC class II were not expressed on neutrophils treated with AEBSF alone. GM-CSF failed to prolong the survival time of neutrophils up to 3 days but increased the expression levels of DC markers on neutrophils in the presence of AEBSF. Expression levels of DC markers were the highest on neutrophils treated with GM-CSF and AEBSF for 3 days. AEBSF and GM-CSF-treated neutrophils stimulated proliferation of T cells in the presence of a superantigen, Staphylococcal enterotoxin B (SEB) but produced interferon-γ (IFNγ) in the absence of SEB. These results suggest that the inhibition of serine protease activity prolonged the life span of human neutrophils and combined treatment of neutrophils with GM-CSF and serine protease inhibitor induced differentiation of neutrophils into DC-like cells.
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KEYWORD
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Trans-differentiation, Apoptosis, Dendritic cells, Neutrophils, Serine protease, AEBSF
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